Fig. 1. A. Effect of oxidative stress on the percentage of annexin V binding erythrocytes. Original histogram and arithmetic means ± SEM (n = 10) of annexin V binding of erythrocytes following exposure to Ringer solution without (black line and white bar) and with (red line and black bar) the presence of 0.3mM tBOOH. ****(p<0.001) indicates significant difference from the absence of tBOOH (ANOVA). B. Effect of oxidative stress on erythrocyte forward scatter. Original histogram and arithmetic means ± SEM (n = 10) of forward scatter of erythrocytes following exposure to Ringer solution without (black line and white bar) and with (red line and black bar) presence of 0.3mM tBOOH. ****(p<0.001) indicates significant difference from the absence of tBOOH (ANOVA). C. Effect of oxidative stress on cytosolic Ca²⁺ concentration. Original histogram and arithmetic means ± SEM (n = 10) of Fluo3 fluorescence reflecting cytosolic Ca²⁺ concentration in erythrocytes following exposure to Ringer solution without (black line and white bar) and with (red line and black bar) the presence of 0.3 mM tBOOH. ****(p<0.001) indicates significant difference from the absence of tBOOH (ANOVA).